Identification of Aneuploidy in Mouse Gliomas Using Whole Genome Sequencing to Determine Large-Scale Copy Number Variations

The aim of this study is to investigate the presence of aneuploidy in mouse gliomas by comparing copy number variations (CNVs) between two different tumor types - H3.3 K27M and H3.3 S31A. To control for CNV differences between the mouse strains and the mouse reference genome, we will perform whole genome sequencing (WGS) of DNA extracted from formalin-fixed paraffin-embedded brain tumors (FFPE) and compare it with the mouse reference genome. We will also establish a combined reference control sample DNA from PFFE brain tissue combined from mice of the same strain. The detection of CNVs will be based on the analysis of the WGS data. This study will provide valuable insights into the molecular mechanisms underlying aneuploidy in mouse gliomas, which could ultimately lead to the development of targeted therapies for this disease.