Cochlear transcriptome after noise trauma and dexamethasone therapy in mice

In order to elucidate molecular mechanisms of noise-induced hearing loss and dexamethasone therapy in the cochlea (inner ear), transcriptome of cochlear samples was analyzed after induction of hearing loss by exposure to intense noise in mice. Dexamethasone was intraperitoneally injected immediately following the noise trauma. Cochlear transcriptome was analyzed at 12h and 24h following the noise trauma and dexamethasone administration. The samples included cochlear RNA from 1) Baseline, neither noise exposure nor dexamethasone, 2) Noise only, 3) 12h after noise and dexamethasone administration (Dex12h), 4) 12h after noise and control saline (Control12h), 5) 24h after noise and dexamethasone administration (Dex24h), and 6) 24h after noise and control saline (Control24h) groups (6 mice for each group).

为阐明耳蜗（内耳）噪声性听力损失及地塞米松（dexamethasone）治疗的分子机制，本研究对经强噪声暴露诱导听力损失的小鼠耳蜗样本的转录组（transcriptome）进行了分析。噪声损伤后即刻对小鼠腹腔注射地塞米松。本研究分别于噪声暴露及地塞米松给药后的12小时与24小时，对耳蜗转录组开展检测分析。本次实验的样本涵盖6组小鼠的耳蜗RNA，各组分别为：1）基线组：未接受噪声暴露与地塞米松给药；2）仅噪声暴露组；3）噪声暴露并地塞米松给药后12小时组（Dex12h）；4）噪声暴露并生理盐水对照给药后12小时组（Control12h）；5）噪声暴露并地塞米松给药后24小时组（Dex24h）；6）噪声暴露并生理盐水对照给药后24小时组（Control24h）。每组包含6只小鼠。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于小鼠耳蜗在噪声创伤和地塞米松治疗后的转录组变化，旨在揭示噪声性听力损失及地塞米松治疗的分子机制。实验设计包括基线、噪声暴露、地塞米松治疗和对照组，在创伤后12小时和24小时进行转录组分析，共涉及6组样本，每组6只小鼠，使用Agilent微阵列平台进行表达谱分析。