Expression data from mouse macrophages after being cultured on different surfaces

The adverse macrophage-mediated immune response elicited by the bioinert surface of polyetheretherketone (PEEK) is responsible for the formation of fibrous encapsulation and inferior osseointegration for PEEK implants in the dental and orthopedic fields. A nanoporous thin film constructed by layer-by-layer technique was deposited on PEEK surface, and it can effectively suppress the acute inflammatory response of macrophages. However, the underlying molecular mechanism why macrophages respond differently on the modified surface is unknown. We used microarrays to elucidate the underlying molecular mechanisms why macrophages respond differently on the modified surface of PEEK. The murine-derived macrophage cell line RAW264.7 was used as the model cells. Cells were seeded on pristine PEEK surface (Control) and modified PEEK surface (Sample) in triplicate and cultured for 3 days. Then the RNA of these cells were extracted and hybridized on Affymetrix microarrays.