OlSiFaComp : A database for Olea: Olive Self-Incompatibility Flower Allelic Composition

Introduction : Data on olive cross and selfing studies were numerous and dispersed in literature. Varieties were list in lines, and each line correspond to one data – bag, pollen test, paternity tests, ...To compare data in bags standardization was achieved based on 100  hermaphroditic flowers.

Materials and methods : Data from publications were recorded keeping the name of origin for each variety. Varieties in pair wise combinations in crosses enabled to decipher the S-allele pair (Breton and Bervillé 2012, Farinelli et al. 2014) leading to attribute the PASI pair. The G group (Saumitou-Laprade et al. 2017) was recorded from Mariotti et al. 2021.

Results : The DSI pair and the PASI pair were introduced and sorting data by screening G1xG2 (1 for fruit) and G2xG1 (1) whereas by screening G1xG1 (0) and G2XG2 (0) show that all crosses display fruit. The DSSM reconciles DSI and PASI to explain Self-incompatibility. Selfing was shown appearing when crosses were 1-0 or 0-1, but not when 1-1, whereas some expected 0-0 combinations lead to fruit. Moreover, paternity tests (column embryo) revealed most of the time a father compatible in DSI , but incompatible in PASI, this is due to DS-D, that shows compatible pollen is insufficient.

The database is useful to check whether the variety has already been studied for SI. It enables to check the homogeneity of cross data in literature.