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ChIP-seq identification of CarD (MSMEG_6077) binding sites in Mycobacterium smegmatis

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NIAID Data Ecosystem2026-05-10 收录
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CarD is essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with CarD-FLAG (LK2539), where an additional copy of CarD under anhydrotetracycline inducible promoter was stably inserted into the genome. CarD-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.

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2025-11-02
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