Comparative Chloroplast Genome Analyses Among Three Sympatric Species of Atraphaxis

DNA was extracted using an improved CTAB method and stored in a refrigerator at -20 ° C. After the DNA samples pass the test, the Covaris ultrasonic crusher is used to randomly break the qualified DNA samples; The preparation of the library was completed through multiple steps such as end repair, addition of A-tail, addition of sequencing connectors, purification, and PCR amplification; After quality inspection, the Illumina Hiseq 2000 platform of Beijing, China was used to sequence the entire genomes of three species, namely Polygonum spinosum, Polygonum frontalis, and Polygonum slender.