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Real-time observation of clickable cyanopeptide synthesis in bloom-forming cyanobacteria Microcystis and Planktothrix

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NIAID Data Ecosystem2026-05-02 收录
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https://www.omicsdi.org/dataset/bioimages/S-BIAD1325
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In this study time-lapse experiments have been performed using pulsed feeding of non-natural amino acids (non-AAs) in order to observe the build up or decline of azide- or alkyne-modified (clickable) microcystins (MCs) or anabaenopetins (APs) in M. aeruginosa and P. agardhii. Clickable MCs or APs have been subsequently labeled via Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) with ALEXA Fluor 488 (A488) and imaged using confocal microscopy analysis
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2025-04-22
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