DUX domain-dependent chromatin accessibility of DUX binding sites

To determine whether DUX domain binding to SMARCC1 influence chromatin reodeling at DUX binding sites, we performed ATAC-seq We know DUX binding sites are open at the time of expression, but we do not know which domains are required to be present with the discovery of the C-terminal repeats and 14 amino acid tail Overall design: After 12 hour expression of mCherry-tagged DUX derivatives, 30 minutes of transposition usina commercial Tn5 was followed by library preparation