Stephenson Covid-19 PBMC 2021 TCR

Dataset published by Stephenson et al. 2021, which contains transcriptome sequencing for over 750,000 Peripheral Blood Mononuclear Cells (PBMC) from 130 patients in the context of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) <br> The dataset was chosen as an example of a large-scale single cell dataset including VDJ-Sequencing, that is well known to the community. It contains over 150,000 B-Cells and 200,000 T-Cells with IR annotation, that we will regard in this analysis tutorial. <br> The dataset available under this link contains the TCR part. Citation Stephenson, E., Reynolds, G., Botting, R.A. <em>et al.</em> Single-cell multi-omics analysis of the immune response in COVID-19. <em>Nat Med</em> <strong>27</strong>, 904–916 (2021). https://doi.org/10.1038/s41591-021-01329-2 Manuscript link https://www.nature.com/articles/s41591-021-01329-2 Original data link https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-10026/E-MTAB-10026.processed.2.zip

本数据集由Stephenson等人于2021年发布，包含来自130名新型冠状病毒（Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2）重症感染患者的超过75万个外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC）的转录组测序数据。
该数据集被选为包含VDJ测序（VDJ-Sequencing）的大规模单细胞数据集的范例，在免疫学界广为人知。数据集内包含超过15万个带有免疫受体（Immune Receptor, IR）注释的B细胞（B-Cell）与20万个T细胞（T-Cell），将作为本分析教程的核心研究对象。
本数据集的T细胞受体（TCR）部分可通过以下链接获取。引用信息：Stephenson, E., Reynolds, G., Botting, R.A. 等. 新冠病毒感染免疫应答的单细胞多组学分析. 《自然·医学》(Nat Med) **27**, 904–916 (2021). https://doi.org/10.1038/s41591-021-01329-2 论文链接：https://www.nature.com/articles/s41591-021-01329-2 原始数据链接：https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-10026/E-MTAB-10026.processed.2.zip