Transcription profiling of HSCs from SYCNRIP WT and KO mice

SYNCRIP depletion results in HSCs losing their self renewal abilities. Next generation sequencing was conducted in SYNCRIP WT and KO HSCs to investigate the transcriptional changes that occur, and result in the loss of self-renewal. SYNCRIP flox/flox conditional knockout mice were developed for this study. Bone marrow cells from these conditional knockout mice were harvested and transplanted into CD45.1 congenic mice, 6 weeks post transplant mice were intraperitoneally injected with polyinosinic:polycytidylic acid (pIpC). 3 weeks following injections, the transplanted HSCs (CD45.2+, Lin-, Sca1+, cKit+, CD150+, CD48-) were FACS sorted and used for RNA-seq.