Co-IP protein identification of WT/MT IVNS1ABP in isogenic iPSCs derived NPCs

We identified a new disease characterized by severe neurological deficits in addition to progeria symptoms. It is caused by IVNSABP gene mutation. The association between IVNS1ABP and aging has never been reported before. By generating isogenic iPSCs from the patients’ fibroblasts and differentiating the iPSCs into neural progenitor cells (NPCs),we performed a pull-down assay with an IVNS1ABP antibody, followed by mass spectrometry to identify the potential protein interactors of both wild type (Ctrl) and MT IVNS1ABP using NPCs from the isogenic pairs.