Data published for 'Protective Effects of Polyphenol-Rich Extracts against Neurotoxicity Elicited by Paraquat or Rotenone in Cellular Models of Parkinson’s Disease' (Tambe et al., Antioxidants 2023)

<p>In the study represented by the uploaded dataset (Tambe et al., Antioxidants 2023), we characterized botanical extracts enriched with a mixed profile of polyphenols, as well as a set of purified polyphenolic standards, in terms of their ability to mitigate dopaminergic cell death in primary midbrain cultures exposed to the Parkinson's disease (PD)-related toxicant, paraquat (PQ), and we examined underlying neuroprotective mechanisms. Extracts prepared from blueberries, black currants, grape seeds, grape skin, mulberries, and plums, as well as several ANC, were found to rescue dopaminergic neuron loss in PQ-treated cultures. Comparison of a subset of ANC-rich extracts for the ability to mitigate neurotoxicity elicited by PQ versus rotenone revealed that a hibiscus or plum extract was only neuroprotective in cultures exposed to rotenone or PQ, respectively. Several extracts or compounds with the ability to protect against PQ neurotoxicity increased the activity of the antioxidant transcription factor Nrf2 in cultured astrocytes, and PQ-induced dopaminergic cell death was attenuated in Nrf2-expressing midbrain cultures. In other studies, we found that extracts prepared from hibiscus, grape skin, or purple basil (but not plums) rescued defects in O<sub>2</sub> consumption in neuronal cells treated with rotenone. Collectively, these findings suggest that extracts enriched with certain combinations of ANC, PAC, stilbenes, and other polyphenols could potentially slow neurodegeneration in the brains of individuals exposed to PQ or rotenone by activating cellular antioxidant mechanisms and/or alleviating mitochondrial dysfunction. The uploaded dataset consists of (i) an Excel worksheet with tabulated data spanning Figures 1-7 and S1-S3; (ii) a set of Prism files showing the results of statistical analyses of the data in Figures 1-7 and S1-S3 (readable with GraphPad Prism software); and (iii) a set of .jpg and .nd2 files corresponding to the fluorescence micrographs presented in Figure S4 (.nd2 files are readable using NIS-Elements Viewer software).</p>