ME3BP-7 is a targeted cytotoxic agent that rapidly kills pancreatic cancer cells expressing high levels of monocarboxylate transporter MCT1

Nearly 30% of Pancreatic ductal adenocarcinoma (PDAC)s exhibit a marked overexpression of Monocarboxylate Transporter 1 (MCT1), offering a unique opportunity for therapy. However, biochemical inhibitors of MCT1 have proven unsuccessful in clinical trials. In this study, we present an alternative approach using 3-Bromopyruvate (3BP) to target MCT1 overexpressing PDACs. 3BP is a cytotoxic agent that is known to be transported into cells via MCT1, but its clinical usefulness has been hampered by difficulties in delivering the drug systemically. We describe here a novel microencapsulated formulation of 3BP (ME3BP-7) that is effective against a variety of PDAC cells in vitro and remains stable in serum. Furthermore, systemically administered ME3BP-7 significantly reduces pancreatic cancer growth and metastatic spread in multiple orthotopic models of pancreatic cancer with manageable toxicity. ME3BP-7 is, therefore, a prototype of a promising new drug, in which the targeting moiety and the cy..., , # ME3BP-7 is a targeted cytotoxic agent that rapidly kills pancreatic cancer cells expressing high levels of monocarboxylate transporter MCT1

## Overview

This repository contains the raw and processed data supporting the results presented in the manuscript eLife-VOR-RA-2023-94488R1.

The datasets include RNAseq expression values, growth assays, immunohistochemistry (IHC) images, in vivo experimental data, and supplementary materials related to the study of MCT1-mediated 3BP activity in pancreatic ductal adenocarcinoma (PDAC) models.

## Data Organization

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### Figure 1: 3BP sensitivity of PDAC cell lines

**Data Files:**

* Figure 1A source data.xlsx: SYBR Green growth assay numerical and normalized data.
* Figure 1B source data.xlsx: RNAseq expression values from CCLE.
* Figure 1B source data for IC50s.pzf: Raw data and IC50 statistical analysis.
* .png files for Figure 1C: Original IHC images extracted from Proscia.

**Variables and Units:**

* Response (% confluence) — %
* Expr...,