(+)ssRNA, (-)ssRNA, lack/presence of poly(A)-tail, segmented/non-segmented genomes Raw sequence reads. Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Vesicular stomatitis virus (VSV), and Oropouche virus (OROV)

We developed a simple protocol which eliminated primer design, reverse transcription, and amplification, enabling real-time detection within 2.5 hours. This could help community to better understand and prepare for emerging infectious challenges due to known or unknown pathogens also time-course analysis of six viruses.