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Kinetic constants of human recombinant wild type-GCK and mutant β-cell GST-GCK fusion proteins.

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Data represent means ± SEM of 3 separate enzyme expressions each tested in duplicate. Note that the Hill coefficient (nH) and the relative activity index (Ia) are unit less. Kcat: GCK catalytic constant; S0.5: affinity constant for glucose; nH: Hill coefficient; Km for ATP: affinity constant for ATP; Ia: GCK activity index. (*)p<0.05, t test; (**)p<0.005, t test.

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2012-06-20
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