Recombinant fHbp sequence variants tested for anti-fHbp mAb reactivity.

*As designated in the fHbp public peptide database, http://pubmlst.org/neisseria/fHbp/. **Variant groups as described by Masignani et al [19]. Proteins in variant group 1 are classified as “sub-family B” by Fletcher et al [9], and proteins in variant groups 2 and 3 are classified as “sub-family A”. The relatedness of the amino acid sequences of fHbp in variant group 1 is illustrated in Figure 2. ¶As described by Beernink and Granoff [20] and Pajon et al [21]. †codon usage was optimized for expression in E. coli based on the amino acid sequence obtained from the fHbp gene from strain M2197 [29]. ††codon usage was optimized for expression in E. coli based on the amino acid sequence obtained from the fHbp gene from strain 19498 (GenBank accession number ACI46928) [31].