mRNA-seq analysis of mouse hepatocellular carcinoma cell lines

There are few well-characterized syngeneic murine models for hepatocellular carcinoma (HCC), which limits immunological studies and the development of immunotherapies for HCC. We previously established an oncogene-induced spontaneous HCC mouse model based on transposon-mediated oncogene (AKT and NRASG12V) insertion into the genome of hepatocytes to induce tumorigenesis. Here is the result of transcriptomic analysis of two pool cell clones (pool #1 and pool #8) and three single cell clones (NHRI-1-F1, NHRI-8-A4 and NHRI-8-C4) from Akt/N-Ras-induced HCC mouse model, also including a commercial murine hepatocellular carcinoma cell line (Hepa 1-6) as control. Overall design: Before cells reaching confluence, total RNA was extracted from each cell clone by using RNeasy mini kit (QIAGEN, Hilden, Germany). The quality of RNA was validated by a Bioanalyzer (Agilent, Santa Clara, CA, USA) and subjected to Welgene Biotech Co., Ltd. for RNA-sequencing. Clean reads of each sample provided by the sequencing Labs were used for downstream bioinformatic analysis.