Images and Tabular Data Supporting: "Aceh Patchouli Oil (Pogostemon cablin) Inducing Apoptosis of HeLa Cervical Cancer Cells Through Modulation of the p-AKT Pathway"

This dataset contains all primary figures and tabular data associated with the research article investigating the anticancer activity of Aceh patchouli oil (Pogostemon cablin) against HeLa cervical cancer cells, with emphasis on apoptosis induction via modulation of the phosphorylated AKT (p-AKT) signaling pathway. The dataset includes the following:
- Figures (Images): Cell Morphology Images (Before and After Treatment); this section contains brightfield microscopy images documenting morphological changes in HeLa cervical cancer cells before (untreated control) and after treatment with Aceh patchouli oil at various concentrations, Cytotoxicity assay results (CCK-8 assay); The bar graph illustrates a dose-dependent reduction in cell viability (expressed as percentage relative to untreated control, %), with the x-axis representing treatment concentrations and the y-axis representing percentage cell viability, Flow cytometry; dot plots (Annexin V/PI staining) depicting apoptotic and necrotic cell populations following treatment with patchouli oil at various concentrations and This section contains flow cytometry data and representative dot plot graphs from Annexin V/Propidium Iodide (PI) dual-staining analysis of HeLa cells treated with Aceh patchouli oil across a range of concentrations. Each dot plot illustrates the distribution of cell populations across four quadrants: viable cells (Annexin V⁻/PI⁻), early apoptotic cells (Annexin V⁺/PI⁻), late apoptotic cells (Annexin V⁺/PI⁺), and necrotic cells (Annexin V⁻/PI⁺). The data demonstrate a concentration-dependent shift in the cell population toward apoptotic phases, confirming the pro-apoptotic mechanism of patchouli oil. Tabular data include the quantified percentage of cells in each phase across all treatment concentrations and controls, presented as mean ± SD with corresponding statistical analysis, Immunofluorescence images of p-AKT and cleaved-caspase 3 localization in HeLa cells following patchouli oil treatment.
- Tables (Tabular Data): Cell viability data (%) at each concentration of patchouli oil. Statistical summary of apoptosis rates (early apoptosis, late apoptosis, necrosis, and viable cells) from flow cytometry analysis.

Experimental Context:
All experiments were conducted in vitro using HeLa cervical cancer cell lines. Patchouli oil was obtained from Aceh province, Indonesia. Treatments were performed across multiple concentrations with appropriate positive and negative controls. All data are presented as mean ± standard deviation (SD) from at least three independent biological replicates.

Intended Use:
This dataset supports transparency and reproducibility of the published findings and is made openly available for use by researchers in the fields of natural product pharmacology, oncology, and cell biology.