Statistical significance tests between conventional and molecular methods (n = 302).

Note: “a” denotes binomial distribution used. Statistical analysis showed 0 cells (0.0%) have expected count less than 5 for all methods. Variation is observed for different methods in terms of the minimum expected counts (MEC), i.e. MEC for “b” is 34.77; “c” is 14.57; “d” is 28.48; “e” is 27.15; “f” is 42.05; “g” is 42.05; “h” is 47.02; and “i” is 41.06. Degree of freedom (df) is one (1) for all calculations. Data is considered statistically significant if p value is less than 0.05.Abbreviations: n: number of patients; %: percentage; H & E staining: Hematoxylin and Eosin; L-J egg medium: Lowenstein Jensen; AFB+Ve: Acid fast bacilli positive; Z-N staining: Ziehl-Neelsen's; PCR: Polymerase Chain Reaction; 19 kDa (131 bp):19 kDa antigen gene; TRC4 (173 bp): TRC4 element; MPB64 (240 bp): MPB64 antigen gene; 32 kDa (506 bp): 32 kDa protein (MTP-59 α-antigen) gene.