Identification of a shared disease-associated oligodendrocyte signature among multiple CNS pathologies

The role of oligodendrocytes in Alzheimer’s disease (AD) has never been fully elucidated. To dissect their possible contribution to the disease we perform single-cell RNA-sequencing (scRNA-seq) on the brains of healthy and 5XFaD (genetic model of AD) at different ages. We performed massively parallel single-cell RNA sequencing (MARS-seq2.0) on non-immune cells (CD45-) or specifically on oligodendrocytes (GalC+) from brains of WT and 5XFaD mice.