Supporting data for "Developmental dynamics of chromatin accessibility during post-implantation development of monkey embryos"

Early post-implantation development, especially gastrulation in primates, is accompanied by extensive drastic chromatin reorganization, which remains largely elusive. To delineate the global chromatin landscape and understand the molecular dynamics during this period, single-cell assay for transposase accessible chromatin sequencing (scATAC-seq) was applied to in vitro cultured monkey embryos to investigate the chromatin status. We delineated the cis-regulatory interactions and identified the regulatory networks and key transcription factors involved in epiblast (EPI), hypoblast, and trophectoderm (TE)/trophoblast lineage specification. We also identified the opposing roles of FGF and BMP signaling in pluripotency regulation in EPI cells. The integrative analysis of chromatin accessibility and gene expression revealed that chromatin status activation precedes gene expression during EPI and trophoblast specification. Finally, we revealed the similarity between EPI and TE in terms of gene expression patterns and demonstrated that NR2F2 and PATZ1 are involved in EPI and trophoblast specification during monkey post-implantation development. Our findings provide new resources and insights into dissecting the transcriptional regulatory machinery during primate post-implantation development.