Transcriptomic and Chromatin accessibility profiling of functional brown adipocytes derived from human pluripotent stem cells

Brown adipocytes (BAs) are a potential therapeutic cell source for the treatment of metabolic disease such as type 2 diabetes. In this report, human pluripotent stem cells (hPSCs) are subject to directed differentiation to brown dipocytes through a paraxial mesoderm intermediate at high-efficiency. RNA-Seq and ATAC-seq was performed to characterized hPSCs derived paraxial mesoderm and brown adipocytes generated in this study. Overall design: RNA-Seq was perfomed on iPSC and hESC derived paraxial mesoderm, iPSC and hESC derived full differentiated brown adipocytes. The differentiation process of brown adipocytes was also interrogated by RNA-Seq profiling at various differentiation time points. Time-course ATAC-Seq profiling was perfomed to interrogate the early differentiation process from paraxial mesoderm to brown adipocytes at early differentiation stages.