RNA-seq Analysis of Kidney versus Splenic CD4+ and CD8+ T cells in MRL/lpr Lupus Mice
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Purpose: To understand the differences in gene expression of lupus T cells in secondary lymphoic organ versus site of organ damage Methods: CD4+ and CD8+ T cells from spleen and kidney of 14-week-old MRL/lpr mice were sorted, and their RNA extracted for deep sequencing, in duplicate. Sequenced reads were aligned to MM9 mouse genome using Star and Bowtie.
创建时间:
2020-04-10



