VirtualStaining Dataset

The enclosed set contains human colon adenocarcinoma Caco-2 cells microscopic images obtained under various imaging conditions with different viable vs non-viable cells fractions. Each field of view is provided in a three-fold representation, including phase-contrast microscopy and two differential fluorescent microscopy images with specific markup of viable and non-viable cells, respectively, produced using two different staining schemes, representing a prominent test bed for the validation of image analysis methods.

本附数据集包含人类结肠腺癌Caco-2细胞（Caco-2 cells）的显微图像，所有图像均采集于多种成像条件下，且涵盖不同活细胞与死细胞比例的样本。每个视野均以三重表征形式提供：包含一张相差显微镜成像图，以及两张分别采用两种不同染色方案获取的差分荧光显微镜成像图，二者分别对活细胞与死细胞进行了特异性标记。该数据集可作为验证图像分析方法的优质测试平台。