PUMILIO Proteins in Colorectal Cancer: Tumor Promoting Function and Potential as Therapeutic Targets. Homo sapiens

We employ photoactivatable ribonucleoside-enhanced crosslinking and Immunoprecipitation (PAR-CLIP) to reveal Pum1 binding mRNAs overall design: To unravel the molecular mechanisms mediated by Pum1, we performed PAR-CLIP to identify Pum1 target mRNAs and to delineate the Pum1 binding sites at the single necleotide resolution. we used human colorectal cancer cell line Hct116 for Pum1 PAR-CLIP, with Pum1 knockout as the negative control. two biological duplicates were carried out for each sample. We employ mRNA-seq to investigate transcriptome of Pum1-Knockout, Pum2-Knockout and WT conditons Overall design: In order to investigate whether Pum1 and Pum2 regulate their targets at their RNA levels, we used Pum1-Knockout, Pum2-Knockout and WT Hct116 cell line to extract total RNAs for RNA deep sequencing.