Three cucumbers genome sequencing and assembly

Three cucumbers were whole-genome sequenced by oxford nanopore technology: EA35 (East Asian cucumber), XIS49 (Xishuangbanna cucumber, Cucumis sativus var. xishuangbannesis), and WI22 (wild Indian cucumber, Cucumis sativus var. hardwickii). We take these three materials as representative cucumbers for cultivated cucumber, semi-wild cucumber and wild cucumber, respectively. Three chromosome-scale genomes were assembled by using the long reads. These works are to undermine new sequential variants/structural variants that control important traits like hypocotyl elongation, flowering time, sex expression, temperature-stress tolerance, fruits traits, and so on. Methylation-related ionic current captured in the ONT sequencing is used to profile the DNA methylation in single nucleotide resolution.