Amazon river Myxozoa Oxford-Nanopore sequencing. Ellipsomyxa sp.

Oxford-Nanopore sequencing, is a PCR-free method that retains epigenetic markers and provides direct quantitative information about DNA methylation. Using this long-read sequencing technology, five myxozoan genomes free from host DNA contamination have been sequenced and their genomes were assembled. These genomes did not match any existing sequences in public databases, suggesting a need for updated sequence data for these elusive parasites. The quality of the assemblies surpassed that of existing assemblies, and methylation analysis indicated the presence of DNA methylation in the GC-rich regions within gene bodies.