Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 1: RNA-seq)

By taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we performed an in-depth comparison of transcriptomic responses in human vaccinees, conventional humanized mice, and second generation humanized mice. We demonstrate that selective expansion of human myeloid and natural killer cells in humanized mice promotes transcriptomic responses akin to those of human vaccinees The transcriptome of human PBMCs from different humanized mouse models (conventional model: 3 cohorts/samples of 5 mice each; NFA2-HIS/Fluc model: 2 cohorts/samples of 4 mice each; NFA2-HIS/Flt3LG model: 3 cohorts/samples of 4 mouse each) was determined by RNA-Seq prior (day 0 post infection; control) and after YFV-17D infection (day 11 post infection; post infection). Resulting data sets of differentially expressed genes were compared to data sets from human vaccinees (GSE13485 and GSE13699). Total number of samples processed for RNA-seq: 16