Transcriptomic and chromatin profiling of familial Alzheimer's disease mutations in PSEN1, PSEN2, APP [ATAC-seq]

Mutations in PSEN1, PSEN2, and APP cause familial Alzheimer's Disease (FAD) with an early age at onset and progressive cognitive decline. We mechanistically characterize mutations in these three FAD genes using patient-derived neurons by integrating RNA- and ATAC-sequencing. Here, we demonstrate that FAD mutations share common disease endotypes with varying severity, particularly activation of non-ectoderm lineage and loss of neuron mitochondrial energy production, paving the way for potential therapeutic interventions. Overall design: Chromatin accessibility (ATAC-seq) profiling of iPSC-derived neurons from healthy donors (NDC) and familial Alzheimer's disease (FAD) patients with APP_V717I, PSEN1_A79V, and PSEN2_N141I mutations