Rhizosphere microbial diversity of red fescue and grapevine with varying root biomass in sample

Few studies examine rhizosphere bacteria from small amounts (low biomass) of root material. We varied the quantity of grass (Festuca rubra) and grapevine (Vitis spp. rootstock 101-14 Mgt.) absorptive roots (1st branching order), used a sonic water bath to remove rhizosphere soil, extracted DNA from the solution and performed high-throughput sequencing of the 16S rRNA gene region.