Ethanol test to evaluate the physiological quality of forest seeds

Abstract: Rapid tests that allow the assessment of physiological potential are essential parameters for quality seed and seedling production programs. The aim of this work was to establish parameters of a method for evaluating the vigor of seeds of native species through the analysis of ethanol production, measured with a modified alcohol meter. Six lots of Myracrodruon urundeuva were tested, and for each lot 1.0 g and 2.0 g of seeds were soaked for two, four, six, eight and 24 hours. Seven lots of Cenostigma pyramidale and five lots of Amburana cearensis were tested, where 20 seeds were soaked for two, four, six, eight and 24 h. The physiological quality of the seeds was also evaluated by germination and electrical conductivity tests. The use of 1.0 g of seeds soaked in 0.5 mL of water and evaluation of ethanol at six hours distinguished lots of M. urundeuva in terms of vigor. For C. pyramidale and A. cearensis, 20 seeds soaked in 1.0 mL of water and ethanol evaluation at six hours proved to be more efficient. The ethanol test proved to be fast and accurate, allowing differentiation of lots, as well as germination and electrical conductivity tests.