Real-time quantitative PCR analysis of Lentinula edodes mycelium face on different stress treatments.. Real-time quantitative PCR analysis of Lentinula edodes mycelium face on different stress treatments.

L. edodes strain w1 was cultured at 25°C in MYG medium (containing 1% malt extract, 0.1% peptone, 0.1% yeast extract, and 2% glucose). Fresh mycelium blocks were cultured in a 9cm petri dish containing MYG medium. After 8-day dark cul- ture, and they were divided into different treatment groups (group A,B,C,D,E). Group A was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then treated at 37 °C for 30 min. 3. Group B was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then treated at 4 °C for 60 min. Group C was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then under 3500 lx light for 1 h. In group D, 8mm-diameter fresh L. edodes mycelium block were inoculated into MYG medium containing cadmium ions. In group E, 8mm-diameter L. edodes mycelium block was inoculated at 10 mm from the edge of the petri dish, and cultured at 25 ° C for 7 days. Afterwards, an activated 8-mm diameter tricho- derma mycelium block was inoculated at 1 cm from the edge of the petri dish at one end of the petri dish diameter (corresponding to the other end where L. edodes mycelium block was inoculated) Overall design: qPCR gene expression profiling. Lentinula edodes mycelium were used and treated separately as indicated in the summary.