A2PP binds to p37 of <i>M</i>. <i>hyorhinis</i> and has minimal effect on the proliferation of gastric cancer cell.

(A) Schematic diagram of amino acid sequence of N-terminal of ANXA2 polypeptide (A2PP). (B) Solid-phase binding assay. OD490 of 15 nM biotin-A2PP to GST was set as 1 and relative bindings were caculated. Mean ± SD of three independent assays with triplicate samples. ***, P < 0.001. (C) Streptavidin pull-down assays identified Biotin-A2PP as a GST-p37 binding polypeptide. (D) Cell morphology of AGS and BGC823 following indicated concentrations of A2PP treatment for 24 hr and 48 hr. (E) Proliferation of gastric cancer cell lines (AGS and BGC823) treated with increasing concentration of A2PP for 72 hr. Mean ± SD from three experiments with triplicate samples.