Ion Torrent RNA-sequencing of exosomes isolated from ovarian cancer cells, fibroblasts and adipocytes

We used next-generation sequencing to identify differential microRNA (miRNA) signatures in exosomes isolated from ovarian cancer cells and ovarian cancer-associated fibroblasts (CAFs) and adipocytes (CAAs). We found that a significantly higher levels of miR21 isomiRNAs in exosomes isolated from CAFs and CAAs than in those from ovarian cancer cells. Overall design: Samples are exosomes isolated from four ovarian cancer cell lines (A2780, HeyA8, OVCA433 and SKOV3), two normal fibroblasts, three CAFs, two normal adipocytes and two CAAs