Single cell transcriptomic profiling of day 30 patient-specific pluripotent stem cell-derived alveolar epithelial type 2 cells expressing the SFTPCI73T mutation and their gene-edited corrected counterparts [D30]

We performed transcriptomic profiling of cells derived from human induced pluripotent stem cells (iPSCs) using our previously described distal lung directed differentiation protocol to generate alveolar epithelial type 2 cells (iAEC2s). We used the SPC2 human iPSC line and specifically the SPC2-ST-B2 (SFTPCtdT/WT) and SPC2-ST-C11 (SFTPCI73T/tdT) clones containing a SFTPCtdTomato knock-in reporter. Live SFTPCtdTomato+ iAEC2s were sorted on day 30 and encapsulated for scRNA-seq using the 10X Chromium System. We find that mutant (SFTPCI73T/tdT) and corrected (SFTPCtdT/WT) iAEC2s have similar transcriptomes with only 403 genes being differentially expressed at this early time point after the initial onset of SFTPC expression. Overall design: Global transcriptomic profiling of day 30 patient-specific induced pluripotent stem cell (iPSC)-derived alveolar epithelial type 2 cells (iAEC2s) expressing the SFTPCI73T mutation and their gene-edited corrected counterparts by scRNA-seq