Measured O₂ affinities (<i>P</i>₅₀) and kinetic O₂ dissociation rates (<i>k</i>_off) (means ± SD; n = 3) and derived O₂ dissociation equilibrium constants (<i>K</i>_d) and apparent kinetic O₂ association rates (<i>k</i><sub>

Linearly extrapolated values at 1.0 SNO/heme ratio and 1.0 NEM/heme ratio are indicated. Apparent kon values are reported for identical degrees of thiol modification. Experimental conditions: 20°C, pH 8.3. 10.6 SNO/heme, from [9]. 20.4 SNO/heme. 30.5 SNO/heme. 40.6 SNO/heme. 50.75 NEM/heme. *Significantly different from the unmodified form (P<0.05), unpaired t-test.