Autosomal SCs are shorter on average in <i>Trip13^mod/mod</i> spermatocytes.

Pachytene spermatocyte spreads were immunostained for SYCP3 and lengths of autosomal SCs were measured from wild-type and Trip13mod/mod spermatocytes. The bivalents in each spread were rank-ordered by length from 1 (largest) to 19 (smallest), then divided into groups of similarly sized chromosomes. Note that each of the chromosomes in each size class makes up essentially the same percentage of total SC length in both genotypes, indicating that the decrease in average total SC length in Trip13mod/mod spermatocytes is uniform across all autosomes. aMean ± standard deviation for each chromosome in the indicated size rank. bThe mean SC length per chromosome divided by the average total SC length per cell, multiplied by 100. cTotal number of chromosomes analyzed. dThese values are statistically significantly different from those for wild type (p≤0.05, t test).