RNA-sequencing data
收藏DataCite Commons2021-03-22 更新2024-07-28 收录
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https://figshare.com/articles/dataset/RNA-sequencing_data/14176463/1
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Mouse lung ECs were isolated as described previously [8,35]. Purified endothelial cells (EC, CD31<sup>+ </sup>cells) were lysed for RNA isolation with the RNeasy mini kit (Qiagen Inc., Germantown, MD, USA) including DNase I digestion. Equal amounts of RNA from ECs isolated from three individual WT or <i>Egln1<sup>Tie2Cre</sup></i> mice were pooled and sequenced with NovaSeq PE150 at Novogene Corporation Inc. (Sacramento, CA, USA) The original sequencing data were trimmed using FASTX and aligned to the reference genome using TopHat2. The differential expression analysis was performed using Cuffdiff software [36].
提供机构：
figshare
创建时间：
2021-03-07
