Genome-wide epigenetic changes of leukemia cell lines in reponse to byrostatin treatment

We report the application of cut&tag technology for high-throughput profiling of histone modifications in leuemia cells in reponse to bryostatin treatment. We generated genome-wide chromatin-state landscape of NALM6, REH and Raji cells. Dual- and tri-methylation of lysine 4 on histone H3 protein indicate genes that are actively expressed/regulated. Tri-methylation of lysine 27 on histone H3 protein marks genes that are negatively regulated or suppressed for expressiom. We compared differential peaks for cells treated with or without bryostatin to demonstrate genes responding to bryostatin treatment on epigenetic level. Overall design: Examination of histone modifications (H3K4me2, H3K4me3 and H3K27me2) in NALM6, REH and Raji cells treated with or without Bryostatin, using cut&tag for epigentic landscape profiling