Identification of the gene profiling of Wt1GFP enriched epicardial cells at different stages of embryonic heart development [H]. Identification of the gene profiling of Wt1GFP enriched epicardial cells at different stages of embryonic heart development [H]

Detailed FACS analyses of enzymatically disaggregated embryonic hearts from Wt1GFPKI mice revealed two distinct types of Wt1GFP-positive cells based on their GFP expression levels: a Wt1GFP bright population (H) and a Wt1GFP dim (M) population. Quantitative RT-PCR analyses for epicardial genes demonstrated an enrichment in epicardial genes in the Wt1GFP bright population (H) Overall design: In order to characterise the gene expression profile of Wt1GFP enriched epicardial cells, RNA from Wt1-GFP bright population (H) sorted cells from heart ventricles was isolated each day from E11.5 to E16.5.