five

Primary and secondary antibodies.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Primary_and_secondary_antibodies_/370044
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(1)Used in CaMKIIα - PSA-NCAM - PV triple immunohistochemistry.(2)Used in SYN immunohistochemistry for light microscopy.(3)Used in multiple-labeling experiments for the detection of anti-CaMKII-α primary antibody when other primary antibody generated in mouse was also being used. The Avidin, NeutrAvidin®, Texas Red® conjugate (1∶200, Molecular Probes) or streptavidin, Alexa Fluor ® 488 conjugate were used before to detect biotin.Abbreviations: CB, calbindin-D28k; CR , calretinin; CaMKII-α, α subunit of the Ca2+/calmodulin dependent protein kinase II; GAD67, 67 kDa isoform of the glutamate decarboxilase enzime, GAD65/67, both 65 and 67 kDa isoforms of the glutamate decarboxilase enzyme; PSA-NCAM, polysialylated form of de neural cell adhesion molecule; PV, parvalbumin; SYN, synaptophysin; VGAT, vesicular γ-aminobutyric acid (GABA) transporter; VGLUT1, vesicular glutamate transporter 1.
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2015-12-02
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