RPRD PROTEINS CONTROL TRANSCRIPTION IN HUMAN CELLS

Regulation of transcription is an essential process that allows the cell to respond to various internal and external signals. RNA Polymerase II (Pol II) activity is controlled by a number of factors which bind to the C-terminal domain (CTD) of its largest subunit, RPB1, and stimulate or supress RNA synthesis. Here, we demonstrate that members of the RPRD family of CTD-interacting proteins, RPRD1A, RPRD1B and RPRD2, act as negative regulators of transcription. We show they form mutually exclusive complexes with Pol II, coordinating this way their roles in transcription control. Our data indicate that the main alterations in gene expression patterns are driven by RPRD2, which results in further consequences during the cellular stress response, cell cycle and cell growth. Investigation the role of RPRD proteins on accumulation of steady-state RNA.