Brassica napus Raw sequence reads
收藏NIAID Data Ecosystem2026-05-02 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP302281
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资源简介：
Total RNA in roots and leaves of Zhongshuang 11 was extracted using the RNAiso Plus kit (Takara, Dalian, China) according to the manufacturer instructions. RNA quality was monitored on 1% agarose gels and further checked spectrophotometrically with a NanoDrop TM 2000 UV-vis Spectrophotometer . cDNA libraries for all treatments (ammonium nitrogen and nitrate nitrogen) and tissues (leaves and roots) were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer instructions. Transcriptome sequencing was performed on an Illumina Hiseq 4000 platform. Raw data was firstly filtered by removing reads containing adapter and multiple unknown bases as well as some low quality reads. Then, the clean data were aligned to the Brassica napus genome using HISAT2 software.
创建时间：
2025-01-17
