Identification of reported substrates of furin and thrombin using Query Sequence Set.

Furin and thrombin cleavage sequences from the natural substrates were extracted and a training set of different types, i.e. octa, tetra and tri peptides were created. The terapeptide sequence indicates P3P2P1P1′ and the tripeptide sequence indicates P3P2P1 residues. The ability of these query sequences to retrieve known/reported substrates was analyzed as described under methods.
*Note that for the sake of clarity the number of proteins and not the number of cleavage sites are reported here.