Additional file 1 of Sputum bacterial load and bacterial composition correlate with lung function and are altered by long-term azithromycin treatment in children with HIV-associated chronic lung disease

Additional file 1: Figure S1. A bar plot of the taxa and their relative abundance of the extraction and sequencing mock controls compared to manufacturer profiles. Figure S2. A scatterplot showing the correlation between samples repeated within a run (WR, n = 74) and between runs (BR, n=28). Figure S3. A scatterplot showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) 16S copies vs final number of reads (A1 and A2), Shannon alpha diversity index (B1 and B2) and age of participant in years (C1 and C2). Figure S4. Ordination plots of showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by their 16S copies. Figure S5. Ordination plots showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by their number of reads. Figure S6. Ordination plots showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by the age of the participant. Figure S7. Rarefaction curves showing number of ASVs detected and 16S copies of samples. Figure S8. Rarefaction curves showing number of ASVs detected and number of reads of samples. Figure S9. Bar plot showing the profiles of biological samples with 100 to

附加文件 1：图 S1. 展示了提取和测序模拟对照与制造商轮廓相比的物种及其相对丰度的柱状图。图 S2. 散点图展示了同一运行内重复的样本（WR，n = 74）以及不同运行间（BR，n=28）的样本之间的相关性。图 S3. 散点图展示了生物样本（n=960）和阴性对照（Primestore，n=43）的 16S 片段数与最终读取数（A1 和 A2）、Shannon α 多样性指数（B1 和 B2）以及参与者的年龄（C1 和 C2）之间的关系。图 S4. 排序图展示了以 16S 片段数为色的生物样本（n=960）和阴性对照（Primestore，n=43）的分布。图 S5. 排序图展示了以读取数为色的生物样本（n=960）和阴性对照（Primestore，n=43）的分布。图 S6. 排序图展示了以参与者年龄为色的生物样本（n=960）和阴性对照（Primestore，n=43）的分布。图 S7. 稀疏曲线展示了检测到的 ASV 数量和样本的 16S 片段数。图 S8. 稀疏曲线展示了检测到的 ASV 数量和样本的读取数。图 S9. 柱状图展示了生物样本的轮廓，读取数在 100 到 ...