Anti-inflammatory properties of deep sea water in-vitro and in-vivo models of inflamed intestinal epithelium

Purpose: This study aimed to determine the effect of deep sea water (DSW) containing minerals on intestinal health, using a cell model and dextran sulfate sodium (DSS)-induced enteritis animal model. Methods: In vivo, the role of DSW was determined in DSS-induced colitis mouse model. Results: In comparison to the DSS group, colon shortening was inhibited, and disease activity and colon injury were suppressed in DSW-treated mice. RNA isolated from colonic tissues of mice in the control and experimental groups was subjected to RNA-seq. Gene expression of inflammatory genes was similar among control and HMLS mice. In the HMLS group, inflammatory pathways were suppressed through reductions in the expression of genes such as TNF-a, Nos2, and inflammatory chemokines. Conclusions: Our findings suggest that DSW intake can help to colitis, and the effects may be partially regulated by magnesium and other minerals. Overall design: Intestinal colitis was induced for one week with DSS from 8-weeks old mice that has consumed deep sea water for three weeks. This is the RNA-seq data shown by intestinal tissue using Illumina 6000.