large DNA fragment enrichment for Nanopore sequencing in region 22q11.2

Accurately identification of the breakpoint contributes to a clearer understanding of the 22q11.2 deletion syndrome . In this study,we present a feasible nanopore sequencing method of 22q11.2 deletion .This DNA enrichment method, region-specific Amplification (RSA), is able to analyze 22q11.2 deletion by specific amplification of approximately 1Mb region where the breakpoint might exist.