Gene expression of the Dnmt1/3a/3b TKO ESCs which ubiquitously express full-length DNMT1(TKO_FL) or a deletion mutant DNMT1 lacking the N-terminal 602 amino acids(TKO_602).

Gene expression analysis of TKO_FL ESCs and TKO_602 ESCs(day 0), differentiated cells from each ESCs(day4 and day7). To form embryoid bodies (EBs), all ES cells were cultured in MEF medium without LIF. EBs were placed in a cell culture plate, and adherent cells were cultured. To induce primitive streak formation, 10 ng/ml Activin A (R&D Systems) was added. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen). Microarray expression analysis was performed using RNA extracted from mouse ESCs and their derivatives.