Characterization of the El Tor binding glycosphingolipid of rabbit erythrocytes.

(A) Chemical detection by anisaldehyde. (B) Autoradiogram obtained by binding of V. cholerae strain JBK 70. The lanes were: Lane 1, total non-acid glycosphingolipids of rabbit thymus, 40 µg; Lane 1, total non-acid glycosphingolipids of rabbit erythrocytes, 40 µg; Lane 3, B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 4, fraction RE-I of rabbit erythrocytes, 2 µg. (C) Base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction RE-I by hydrolysis with endoglycoceramidase II. (D) MS2 spectrum of the [M-H+]− ion at m/z 868 (retention time 14.0 min). (E) MS2 spectrum of the [M-H+]− ion at m/z 1030 (retention time 14.7 min). (F) Anomeric region of the 600 MHz 1H NMR spectrum of fraction RE-I (30oC). The designations A and D refer to Table 1.