Synchronized HTC116 cells: time course. Homo sapiens

Analysis of synchronized HCT116 cells at various time points up to 10 hours following treatment with DMSO or Nocodazole. Overall design: HCT116 colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC) and grown in McCOY’S 5A MEDIUM MODIFIED (SIGMA) with 10% FBS (JBS). It was maintained at 37°C and 5% CO2. Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9 h-incubation in normal medium and a second 16 h-incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9 and 10 h as a control or 0.1 mg/ml Nocodazole (Sigma) for 7, 8, 9 and 10 h.